Assessment of cytochrome P450 1A in harbour seals (Phoca vitulina) using a minimally-invasive biopsy approach     

Miller KA  Assunção MG  Dangerfield NJ  Bandiera SM  Ross PS 《Marine environmental research》2005,60(2):153-169

Biomarkers of organochlorine exposure, such as the induction of cytochrome P450 1A (CYP1A), can be used to assess the impact of environmental contaminants on the health of free-ranging marine mammal populations. The objective of the present study was to measure CYP1A in skin and liver biopsies obtained from live harbour seals (Phoca vitulina). Twelve harbour seal pups, aged three to five weeks, were captured from the Fraser River estuary, British Columbia, Canada, and temporarily held in captivity. Skin ( approximately 60 mg) and liver ( approximately 40 mg) biopsies, obtained while seals were under general anaesthesia, yielded sufficient tissue for the measurement of CYP1A by immunoblot analysis and ethoxyresorufin O-deethylase activity. A short-term exposure experiment, in which harbour seals (n=3) were treated orally with beta-naphthoflavone (BNF), resulted in increased hepatic and cutaneous CYP1A protein levels, consistent with observations in other mammals. This study is the first to measure CYP1A in skin and liver biopsies from live harbour seals and to report in vivo BNF-associated CYP1A induction in a marine mammal. The results demonstrate that microsamples collected using minimally-invasive techniques can provide toxicologically-relevant information form marine mammals.  相似文献   

The Sponge Population of the Cabrera Archipelago (Balearic Islands): Characteristics, Distribution, and Abundance of the Most Representative Species   总被引：2，自引：0，他引：2  

María Jesús  Uriz  Dolors  Rosell Daniel  Martín 《Marine Ecology》1992,13(2):101-117

Abstract. The abundance, distribution, and species richness of the littoral sponges (0–55 m) from the Cabrera Archipelago (Balearic Islands) were studied along light gradients from the surface to the sea bottom and from cave mouths to their inner parts. A total of 98 species (11 Calcarea and 87 Demospongiae ) were recorded, 8 of which are new records for the Spanish coast.
Cluster and correspondence analyses enabled the identification of a sciophilous sponge group including three subgroups: sponges found only in semi-dark cave habitats, a subgroup common to the coralligenous, precoralligenous, and semi-dark cave biocoenoses, and a third subgroup exclusive to the coralligenous biocoenosis. A small number of photophilic species and other species having a wide bathymetric distribution made up another separate group. No species clusters for the different semi-sciophilous (either fleshy or carbonate) or photophilic seaweed communities were discernible. The number of sponge species was negatively correlated with the irradiance level.  相似文献   

Effect of Sea Level Variation on Tidal Characteristic Values for the East China Sea     

于宜法  俞聿修  左军成  万振文  陈宗镛 《中国海洋工程》2003,17(3)

1 .IntroductionTheglobalairtemperatureroseabout 0 .5～ 0 .6°Coverthepast 2 0thcentury ,andtheglobalmeansealevelincreasedbyabout2 0cmduringtheperiod .Theregionalmeansealevelriseswiththerisingglobalmeansealevel.Zuoetal.( 1 997)indicatedthatthemeanrisingrateofabsolutemeansealevelalongtheChinacoastontheassumptionofunifiedisostaticdatumis 2mm a .Woodworth( 1 999)analyzedsealevelspanning 1 76 8tothepresentinLiverpool,andobtainedaseculartrendforheperiodupto 1 880of0 .39± 0 .1 7mm a ,andatrendfort…  相似文献   

Mid-Holocene evolution and paleoenvironments of the shoreface–offshore transition, north-eastern Argentina: New evidence based on benthic microfauna     

Cecilia Laprida  Natalia García Chapori  Roberto A. Violante  Rosa H. Compagnucci   《Marine Geology》2007,240(1-4):43-56

A sedimentary record spanning 5792–5511 cal yr BP and 3188–2854 cal yr BP was recovered at 36° 45′ 43″ S–56 ° 37′ 13″ W, south-west South Atlantic. The sedimentological features and micropaleontological (benthic foraminifera and ostracoda) content were analyzed in order to reconstruct paleoenvironmental conditions. Considerable environmental fluctuations are indicated by all these proxies. Five different stages were distinguished: Stage 1 (ca. 5800–5000 cal yr BP) consists of muddy sand with abundant microfossils. In this interval, species typical for inner marine shelf environments maintained a high abundance. Stage 2 consists of plastic light greenish grey clays barren of microfossils, and probably represents fluvial input from the de la Plata River to the shelf contemporaneous of a lowering of sea level. Stage 3 is composed of brownish yellow sandy silts, and represents increasing marine conditions in the area as reflected by higher faunal diversity and typical foraminifera of inner shelf environments. Stage 4 is made of homogeneous mud, barren of microfossil, which represents a new pulse of fluvial input to the shelf in consequence of a new fall in sea level. The final part of the core (Stage 5) is a coarsening upward sequence, grading from greeny brown clayey sandy silts to coarse shelly sands and represents the modern sedimentation in the area. This interpretation strengthens the stepped model of late-Holocene sea-level fall between 5511–5792 cal yr BP and 2854–3188 cal yr BP in Buenos Aires coast, and agrees with the relative sea-level history previously proposed by some authors from western South Atlantic coasts.  相似文献   

Stratigraphic framework of gas accumulations in the Ría de Pontevedra (NW Spain)     

Ruth Durán  Soledad García-Gil  Raquel Diez  Federico Vilas 《Geo-Marine Letters》2007,27(2-4):77-88

Knowledge of marine geological environments in which shallow gas is accumulating is becoming increasingly important in global studies of climate change because a measurable proportion of the total methane source comes from continental margins. Previous studies have revealed that coastal environments represent important geological environments where microbial methane is being generated, is accumulating, and is being released. In the Ría de Pontevedra, at least 4.5 km² of seafloor in the innermost part of the ría is underlain by sediments containing natural gas. Seismic interpretation contributes new findings for the definition of periods and geological environments in which the gas could have been generated, and is accumulating and released in the Ría de Pontevedra. Groundtruthing the seismic data (facies, environments) makes it possible to identify favourable geological environments for gas generation in the sedimentary infill of the Ría de Pontevedra. Sequence stratigraphy based on high-resolution seismic profiles and post-Last Glacial Maximum sea-level records makes it possible to establish the stratigraphic architecture of the ría and to define the periods in which gas could have been generated. The results of this study show that the sedimentary infill is composed of a fifth-order sequence developed since the Last Glacial Maximum. Within this sequence, gas appears to have accumulated in the Holocene deposits associated with the latest transgressive and highstand system tracts. Seismic analysis shows that gas could have been generated in different geological environments in the Ría de Pontevedra. If coastal environments at times of lower sea level were similar to those of the present, organic-rich mud deposits (deposited mainly in lakes, estuaries and floodplains) could have survived transgression and remained buried as potential gas sources in the inner part of the ría.  相似文献   

A comparison of Hα and soft X-ray characteristics of spotless and spot group flares     

V. Ruždjak  B. Vršnak  A. Schroll  R. Brajša 《Solar physics》1989,123(2):309-316

A comparative analysis of spotless and spot group flares recorded at Hvar and Kanzelhöhe Observatories during the 21st cycle of solar activity is presented. The rate of occurrence of two-ribbon flares was found to be significantly higher for the spotless flares. In comparison with spot group flares of corresponding H importance, the soft X-ray peak values have been systematically lower for the spotless flares. The highest peak values and the energy released in soft X-rays was found for flares with a H ribbon protruding over a major spot umbra. It was found that the effective plasma temperatures in spotless flares have been considerably lower than the temperatures in spot group flares.  相似文献   

Spin rate estimation of sounding rockets using GPS wind-up   总被引：2，自引：1，他引：1  

M. García-Fernández  M. Markgraf  O. Montenbruck 《GPS Solutions》2008,12(3):155-161

Carrier phase wind-up is a well-known effect that arises from the relative rotation between a transmitting and receiving antenna. In GPS measurements at L1 frequency, this effect translates into an error of 19.029 cm per full relative rotation of antennas. Since this effect is independent of the satellite elevation for pure rotation about the antenna boresight axis, it is usually absorbed by the clock estimation in navigation algorithms. Therefore, the impact of wind-up is usually neglected for applications that do not require accuracies to the cm level like RTK. However, in receiving platforms with high rotation rate, the accumulated wind-up value can be important and actually be larger than receiver noise or even ionospheric variations. Therefore, in such scenarios, the wind-up contribution can be isolated and used as a source of information to compute the spin rate of such platforms using an appropriate combination of GPS observables. This work shows some results of a coarse, yet simple, approach to monitor the rotation angle and spin-rate of spin stabilized sounding rockets flown by DLR.  相似文献   

Presence of Hippidion at two sites of western Argentina: Diet composition and contribution to the study of the extinction of Pleistocene megafauna     

Alejandro García  Eduardo Martínez Carretero  María A. Dacar   《Quaternary International》2008,180(1):22

The composition of Hippidion diet, and possible changes that could relate to its extinction, were evaluated in the Argentinean-Chilean Central Andes, a Neotropical environment characterized by arid to semiarid conditions (Andean hot and cold deserts). Microhistological analyses were made on feces of Hippidion found at Los Morrillos (31°43′S–68°42′W, 3000 m a.s.l.) and Gruta del Indio (34°35′S, 68°22′W, 660 m a.s.l.). At Gruta del Indio the diet of Hippidion was based mainly on woody species. At Los Morrillos, it was based on herbaceous species.This flexibility in diet composition could be a relative adaptive advantage allowing a longer permanence of this species in comparison to others recorded in the region (such as Megatheriumand Mylodon). Nevertheless, this advantage was not enough to guarantee its survival during the Holocene. Extinction could have been affected by diverse agents, such as growing aridification of the area, increasing competition with other species (mainly Lama guanicoe), and human presence, along with a relatively low population density (as expected from the limited presence of Hippidion at the archaeological and palaeontological sites of South America. At Gruta del Indio significant changes in the diet of Hippidion corresponding to different intervals of the period 31,000–9000 ¹⁴C BP are not evident. Given this evidence for similar diets for Hippidion throughout the late Quaternary, other factors need to be considered to explain the extinction of this horse.  相似文献   

Simulations of 3D DC borehole resistivity measurements with a goal-oriented <Emphasis Type="Italic">hp</Emphasis> finite-element method. Part II: through-casing resistivity instruments     

D. Pardo  C. Torres-Verdín  M. Paszynski 《Computational Geosciences》2008,12(1):83-89

We simulate direct current (DC) borehole resistivity measurements acquired in steel-cased deviated wells for the assessment of rock formation properties. The assumed data acquisition configuration considers one current (emitter) and three voltage (collector) electrodes that are utilized to measure the second difference of the electric potential along the well trajectory. We assume a homogeneous, 1.27-cm-thick steel casing with resistivity equal to 10^− 5 Ω· m. Simulations are performed with two different numerical methodologies. The first one is based on transferring two-dimensional (2D) axisymmetric optimal grids to a three-dimensional (3D) simulation software. The second one automatically produces optimal 3D grids yielded by a 3D self-adaptive goal-oriented algorithm. Both methodologies utilize high-order finite elements (FE) that are specially well-suited for problems with high-contrast coefficients and rapid spatial variations of the electric field, as it occurs in simulations that involve steel-cased wells. The method based on transferring 2D-optimal grids is efficient in terms of CPU time (few seconds per logging position). Unfortunately, it may produce inaccurate 3D simulations in deviated wells, even though the error remains below 1% for the axisymmetric (vertical) well. The method based on optimal 3D grids, although less efficient in terms of CPU time (few hours per logging position), produces more accurate results that are validated by a built-in a posteriori error estimator. This paper provides the first existing simulations of through-casing resistivity measurements in deviated wells. Simulated resistivity measurements indicate that, for a 30° deviated well, measurements in conductive layers 0.01 Ω· m) are similar to those obtained in vertical wells. However, in resistive layers (10,000 Ω· m), we observe 100% larger readings in the 30° deviated well. This difference becomes 3,000% for the case of a 60° deviated well. For this highly-deviated well, readings corresponding to the conductive formation layer are about 30% smaller in magnitude than those in a vertical well. Shoulder effects significantly vary in deviated wells.  相似文献   

Water balance in the playa-lakes of an arid environment,Monegros, NE Spain   总被引：1，自引：0，他引：1  

Carmen Castañeda  Miguel Ángel García-Vera 《Hydrogeology Journal》2008,16(1):87-102

The playa-lakes of the Monegros desert in north-east Spain are saline wetlands in an arid environment, a rare phenomenon in Europe. These extremely valuable habitats are threatened by changes associated with agricultural expansion and incorporation of new irrigated areas. An understanding of the present hydrologic regime will enable changes to be identified, particularly those brought about by flooding and pollution caused by irrigation surplus. This study sets out to show the results of applying a daily water balance in three selected playa-lakes. The balance was in two parts and consisted of: (1) the average balance for all the endorheic basin using the BALAN_11 program, and (2) the water balance in some playa-lakes, applying discharge flows obtained from the previous balance. The resulting volumes of water were converted to water depths and contrasted with reference volumes taken from field and Landsat images. The model was calibrated by applying various hypotheses of function which enabled the results to be adjusted. The proposed balance is an acceptable reproduction of field water measurements during this period, and underlines the consistency of the conceptual model. The methodology used is appropriate for understanding the playa-lakes function and for monitoring them for conservation purposes.  相似文献